| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 81 | Thermus thermophilus | TTCCTCGGCACCTACAGGA | AAGGAGGCTGAACCCCATG | 59.92 | 59.31 | 159 | 64 |
100.00%
|
100.00%
|
| Primer 82 | Thermus thermophilus | AGGAGCTCTACGAGGCCTT | GAGGTTCGTGTAGCCCTTCC | 59.69 | 60.11 | 227 | 63 |
100.00%
|
100.00%
|
| Primer 83 | Thermus thermophilus | AGGAGCTCTACGAGGCCTT | CCCTCCACGTTGTAGAGGT | 59.69 | 58.33 | 241 | 63 |
100.00%
|
100.00%
|
| Primer 84 | Thermus thermophilus | AGGAGCTCTACGAGGCCTT | ACGGCAGTAGAGGTAGACGG | 59.69 | 60.75 | 167 | 63 |
100.00%
|
100.00%
|
| Primer 85 | Thermus thermophilus | AGGAGCTCTACGAGGCCTT | ACGGCAGTAGAGGTAGACG | 59.69 | 58.22 | 167 | 63 |
100.00%
|
100.00%
|
| Primer 86 | Thermus thermophilus | AGGAGCTCTACGAGGCCTT | AGAGGTTCGTGTAGCCCTTC | 59.69 | 59.10 | 228 | 63 |
100.00%
|
100.00%
|
| Primer 87 | Thermus thermophilus | AGGAGCTCTACGAGGCCTT | CCCTCCACGTTGTAGAGGTTC | 59.69 | 60.07 | 241 | 63 |
100.00%
|
100.00%
|
| Primer 88 | Thermus thermophilus | AGGACCTCCTCTTCGCCAA | TGGTAGGTGAAGCTCCCCA | 60.23 | 59.84 | 187 | 63 |
100.00%
|
100.00%
|
| Primer 89 | Thermus thermophilus | GCATGGGCTTCTGGGTCTT | CGCATGAGGAACCAGTGCA | 60.00 | 60.67 | 181 | 63 |
100.00%
|
100.00%
|
| Primer 90 | Thermus thermophilus | AGAGGCTCGTGGGGATCAT | GTGGCGATGGAGACGATGT | 60.07 | 59.86 | 169 | 62 |
100.00%
|
100.00%
|